Core unit “Genome signatures and integrated systems biology of pathogen-host interaction and cellular networks in the infected and injured lung”
Understanding cell-specific gene regulatory events and their impact on cellular networks in infected lungs requires an integrative platform which delivers primary lung cells/tissue including highly purified cell populations even of rare subsets, high-throughput genomic and cutting edge epigenetic analyses as well as advanced data processing for bioinformatic exploitation. As such, Z1 provides genomics-based analyses of primary murine and human lung specimens as well as of patient samples to all projects, on a tissue-/cell type-specific and rare/single-cell level. This includes microarray and NGS analyses, as well as storing, bioinformatics processing and integrative modeling of acquired data from experimental, pre-clinical and clinical studies. In long term, it is envisioned to establish algorithms to systematically integrate complex datasets acquired in all projects for multi-component disease and target modeling as well as biomarker identification in the specific context of virus-induced lung injury.
To provide unrestricted access to the rapidly evolving methodology of chromatin profiling of scarce cell subsets from clinical samples, the CRU infrastucture will be further extended by an epigenetics unit supervised by M. Looso and T. Braun (P8). This unit is dedicated to cutting edge epigenetic landscape modeling of flow-sorted human cell subsets from BALF or blood samples to assess multiple layers of chromatin regulation and allow deep phenotyping of human cells in a disease-specific context. This unit will be available to all CRU-associated clinicians and, in long term, will be implemented as permanent facility associated to the Department of Medicine II, to provide epigenetic profiling of rare cells derived from patients with different entities of lung or infectious diseases, aiming at future development of more personalized treatment approaches within a precision medicine concept.